BF-170

  • 详细信息
  • 询价记录
  • 相关实验
  • 货号: 026-16371
    保存条件: -20℃冷冻
    供应商: Wako(和光纯药工业株式会社)
    保质期: 60个月
    规格: 1mg

    for Alzheimer’s Disease (AD)

    • BF-168 (Wako Cat. #029-16361): Senile plaque-selective fluorescent probe
      BF-168 is a probe for senile plaques (SPs). It selectively binds SPs in AD brain sections and recognizes Aβ 1-42 positive diffuse plaques.
    • BF-170 (Wako Cat. #026-16371): Neurofibrillary tangle change-selective fluorescent probe
      BF-170 is a probe for neurofibrillary tangles (NFTs). It exhibits greater binding affinity to Tau fibrils such as NFTs, neuropil threads and paired helical filament than to Aβ fibrils.

     

    B-170

    Solubility: Soluble in methanol and ethanol.
    λex: 450 ~ 490 (nm)
    λem: > 520 (nm)

    Procedure:

    1. Deparaffinize the section on 1 slide glass plate.
    2. Immerse the section with 10% formalin neutral buffer for 60 minutes.
    3. Wash the section with PBS for 5 minutes.
    4. Blanch the section with 0.25% potassium permanganate solution for 20 minutes.
    5. Wash the section twice with PBS for 2 minutes.
    6. Immerse the section with 0.1% potassium metabisulfite and 0.15% oxalic acid for seconds.
    7. Wash the section three times with PBS for 2 minutes.
    8. Immerse the quenched tissue section with 100 μmol/L BF-170 solution
      containing 50% ethanol for 10 minutes.
    9. Dip the section 5 times with water for a few seconds.
    10. Observe the section with fluorescence microscope.

    References:

    1. Kitamoto, T., Ogomori, K., Tateishi, J., and Prusiner, S. B.: Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids., Lab. Invest., 57, 230-236 (1987)
    2. Okamura, N., et al.: Styrylbenzoxazole derivatives for in vivo imaging of amyloid plaques in the brain, J. Neurosci., 24, 2535-2541 (2004)
    3. Okamura, N., Kudo, Y., et al.: Quinoline and benzimidazole derivatives: candidate probes for in vivo imaging of tau pathology in Alzheimer’s disease, J. Neurosci., 25, 10857-10862 (2005)
    温馨提示:不可用于临床治疗。

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