用于测定谷类植物和麦芽中的β-淀粉酵素的试剂盒(RACI标准法) β-amylase Assay (Betamyl-3) 货号:K-BETA3 Megazyme中文站

For the specific measurement of β-amylase in malt flour. Content: 100/200 assays per kit
 

Colourimetric method for the determination of β-Amylase in
cereal grains, malt, food, beverages and fermentation products

Principle:
(β-amylase)
(1) G3-β-PNP + H2O → G2 + G-β-PNP

(β-glucosidase)
(2) G-β-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) p-Nitrophenol → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size: 100 / 200 assays
Method: Spectrophotometric at 400 nm
Reaction time: ~ 10 min
Detection limit: 0.05 U/mL of sample solution
Application examples:
Cereal flours, malts and other materials
Method recognition:
Modification of RACI (Standard Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years as supplied
  • Only enzymatic kit available
  • Very specific
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included