分析物意义： 快速消化和吸收的糖及膳食纤维
Megazyme检测试剂盒优点：检测程序新颖、试剂稳定
An integrated procedure for the measurement of available carbohydrates and dietary fibre in cereal products, fruit and vegetables and food products. Content:100 assays of each component
An integrated procedure for the measurement of Available
Carbohydrates and Dietary Fiber in cereal products, fruit and
vegetables and food products
Principle (Dietary Fiber):
(α-amylase + amyloglucosidase)
(1) Starch + H2O → glucose
(protease)
(2) Protein + H2O → peptides
(3) Dietary fiber determined gravimetrically following
alcohol precipitation
Principle (Available Carbohydrates):
(sucrase / maltase + β-galactosidase)
(4) Sucrose, maltose and lactose → D-glucose + D-fructose
+ D-galactose
(PGI, hexokinase + glucose-6-phosphate dehydrogenase)
(5) D-Glucose + D-fructose + ATP + NADP+ → gluconate-6-phosphate
+ NADPH + ADP + H+
Kit size: 100 assays of each
Application examples:
Food ingredients, food products and other materials
Method recognition:
Dietary Fibre - AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
and AACC (Methods 32-05.01, 32-07.01 and 32-21.01)
Advantages
- Very cost effective
- All reagents stable for > 2 years after preparation
- High purity / standardised enzymes employed
- Only kit available
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Simple format