分析物意义:非常常见的食物糖分,如从高果糖玉米种提炼的糖浆增补剂
Megazyme检测试剂盒优点:反应时间快,可选择简单的版式,适用于手工和自动分析仪检测。试剂稳定
D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of D-Fructose and D-Glucose
in foodstuffs, beverages and other materials
Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(4) F-6-P ↔ G-6-P
Kit size: 110 assays (manual) / 1100 (microplate)
/ 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 13 min
Detection limit: 0.66 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods,
bread, bakery products, candies, desserts, confectionery, ice-cream,
fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals,
paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC