果聚糖[HK法]检测试剂盒 Fructan HK Assay Kit 货号：K-FRUCHK Megazyme中文站

发表于由

分析物意义：许多食品如洋葱和种子中的常见组分

Megazyme检测试剂盒优点：方法新颖、反应快、试剂稳定 

The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharide.

UV-method for the determination of Fructan in foodstuffs,
beverages and other materials

Principle:
(sucrase + maltase)
(1) Sucrose + maltosaccharides + H2O → D-glucose + D-fructose

(exo-inulinase + endo-inulinase)
(2) Fructan + H2O → D-glucose + D-fructose

(hexokinase)
(3) D-Glucose + D-fructose + ATP → G-6-P + F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(5) F-6-P ↔ G-6-P

Kit size:                             50 assays 
Method:                             Spectrophotometric at 340 nm
Total assay time:               ~ 90 min
Detection limit:                  1-100% of sample weight
Application examples: 
Flours, plant materials (e.g. onion), food products and other materials
Method recognition:     
This method is a modification of AOAC Method 999.03 and AACC
Method 32-32.01

Advantages

  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Fructan kits are available only from Megazyme
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included